Next Generation Sequencing

Next- Generation DNA sequencing Review Article By SITA DAHAL dodge of contents Table of Contents2 Abstract:3 Introduction:3 Different sequencing Technologies and Data accumulation:4 SEQUENCING BY synthesis:4 SEQUENCING BY HYBRIDIZATION AND LIGATION:4 SINGLE blood corpuscle SEQUENCING:5 Assembly of Huge Volume of Data from NGS:7 Applications:7 Discussion:7 Conclusion:9 position of reference list:10 Abstract: The topic, DNA sequencing has been at hype from the remnant two decades. This review tries to unravel the conglomerate facet of this engineering and its wide range of applications not still in biomedical research but also in another(prenominal) atomic number 18as. at that place have been rapid technological advancements devising most of the broadcast obsolete. At present, second generation sequencers be being replaced by third generation sequencers having tenacious orders. The sizeableness of bioinformatics and various other questions like where will such large amount of info lead us be dealt. We conk out by this lease that completion of human genome sequencing was not the block but bonnie a starting point to personalised medicine.

Genome sequencing has helped to develop new-sprung(prenominal) vaccines and the drastic reduction in cost of sequencing computer programme has make it possible to be used as clinical symptomatic instrument in near future. Introduction: In 1977, Fredrick Sanger demonstrable the first sequencing technique known as image margin mode. This procedure utilizes dideoxynucleoside triphosphates (ddTTP) which are labeled with hot phosphate. The incor! poration of ddTTP (lacks 3 OH group) terminates the template directed synthesis of the complementary strand. The strands are denatured, size fractioned on four different lanes and the base sequences are read by auto radiography (Metzker, M.L., 2010). Using this procedure, phage ?X 174 was first sequenced genome. It is a less error-prone method and produces long reads of approximately 700 bases but endorses various drawbacks like...If you want to piddle a full essay, order it on our website: BestEssayCheap.com

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